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Key Documents

Safety Information

ABS2090

Sigma-Aldrich

Anti-ICDH

serum, from rabbit

Synonym(s):

Isocitrate dehydrogenase, ICDH (NADP), IDH

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

E. coli, Legionella pneumophila

packaging

antibody small pack of 25 μL

technique(s)

western blot: suitable

isotype

IgG

GenBank accession no.

shipped in

ambient

target post-translational modification

unmodified

General description

Isocitrate dehydrogenase [NADP] (also known as IDH, ICDH) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. In eukaryotes there are at least three isozymes of IDH: two are located in the mitochondrial matrix (one NAD+-dependent, the other NADP+-dependent), while the third one (also NADP+-dependent) is cytoplasmic. In E.coli the activity of a NADP+-dependent ICDH is controlled by the phosphorylation of a serine residue and the phosphorylated form of ICDH is completely inactivated. It has been reported that if Ser113 is replaced by alanine, cysteine, threonine, or tyrosine there is some loss of enzymatic activity. However, substitution by aspartic acid completely abolishes its activity. ICDH contains three domains: a large alpha + beta domain, a small alpha/beta domain and an alpha/beta clasp-like domain involving both subunits. (Ref.: Hurley, JH et al (1989). Proc. Natl. Acad. Sci. USA. 86 (22); 8635-8639).

Specificity

Expected to react with most eubacteria.
This polyclonal antibody detects isocitrate dehydrogenase in E. coli and Legionella pneumophila.

Immunogen

Epitope: unknown
His-tagged full length recombinant isocitrate dehydrogenase from Legionella pneumophila, strain Lp02.

Application

Anti-ICDH, Cat. No. ABS2090, is a rabbit polyclonal antibody that detects Isocitrate dehydrogenase [NADP]. It has been tested for use in Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected ICDH in 20 µL Legionella cell lysate.

Western Blotting Analysis: A representative lot detected ICDH in E. Coli cell lysate (Xu, L., et. al. (2010). PLoS Pathog. 6(3):e1000822; Lu, C., et. al. (2016). Sci Signal. 9(412):ra11).

Quality

Evaluated by Western Blotting in E.Coli cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected ICDH in 20 µL E.Coli cell lysate.

Target description

~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Rabbit polyclonal antiserum with 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ABS2090:
ABS2090-25UL:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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