ABF212

Anti-CIITA Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): MHC class II transactivator, MHC2TA, C2TA, NLRA, CIITAIV
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• technique(s): immunohistochemistry: suitable; western blot: suitable
• UniProt accession no.: P33076
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CIITA(4261)

Description

General description

CIITA, also known as MHC class II transactivator (MHC2TA), is located in the nucleus and is an essential factor for transcriptional activity of the HLA class II promoter. CIITA is suggested to be a positive regulator of class II major histocompatibility complex gene transcription and is referred to as the "master control factor" for the expression of these genes. Additionally, CIITA contains an acidic transcriptional activation domain, four LRRs (leucine-rich repeats) and a GTP binding domain. CIITA binds with GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind with DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Furthermore, it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. CIITA has been shown to interact with ZXDA and ZXDC. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction.

Immunogen

KLH-conjugated linear peptide corresponding to human CIITA.

Application

Detect MHC class II transactivator using this rabbit polyclonal antibody, Anti-CIITA Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: 10 µg/mL from a representative lot detected CIITA in human brain tissue.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CIITA in 15 µg of mouse brain tissue lysate.

Target description

~122 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable