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Safety Information

C3367

Sigma-Aldrich

Anti-CIITA antibody, Mouse monoclonal

clone 7-1H, purified from hybridoma cell culture

Synonym(s):

Anti-C2TA, Anti-MHC Class II Transactivator

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About This Item

MDL number:
MFCD01864254
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7-1H, monoclonal

form

buffered aqueous solution

mol wt

antigen ~130 kDa

species reactivity

mouse, human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
western blot: 2-4 μg/mL using total cell extract of Raji cells

isotype

IgG1

UniProt accession no.

P33076

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CIITA(4261)
mouse ... Ciita(12265)

General description

Class II transactivator protein (CIITA) protein contains an acidic region in its N-terminal with transactivation activity, that binds to proteins in the basal transcription machinery and to cAMP response element-binding protein (CREB)-binding protein (CBP). The protein contains motifs for nuclear translocation, GTP binding motif and C-terminal leucine-rich repeats. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ .
Monoclonal Anti-CIITA (mouse IgG1 isotype) is derived from the hybridoma 7-1H produced by the fusion of mouse myeloma cells (Sp20 cells) and splenocytes from BALB/c mice immunized with purified N-terminal recombinant CIITA, amino acids 1-350. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ.

Monoclonal Anti-CIITA recognizes human1 and mouse † CIITA.

Application

The antibody may be used in immunoblotting (∼130 kDa) and immunocytochemistry.

Biochem/physiol Actions

Class II transactivator protein (CIITA) interacts with specific transcription factors and creates the appropriate transcriptional scaffold required for HLA-D gene expression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C3367-200UL:
C3367-VAR:
C3367-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Giovanna Barbieri et al.
International immunology, 14(8), 839-848 (2002-07-31)
The class II transactivator (CIITA) controls both the constitutive and IFN-gamma inducible expression of HLA-D genes. In addition, through the squelching of another transactivator CREB-binding protein, CIITA was more recently shown to have a wider cellular function, including cell cycle
H Zhou et al.
Journal of immunology (Baltimore, Md. : 1950), 158(10), 4741-4749 (1997-05-15)
The MHC class II transactivator gene (CIITA) coordinately controls the expression of the three major human class II genes, HLA-DR, HLA-DQ, and HLA-DP. Indeed, patients with one form of MHC class II immunodeficiency disease, due to defective CIITA genes, lack
Isoforms of the class II transactivator protein
Barbieri G, et al.
International Immunology, 14(8), 839-848 (2002)
W Wiszniewski et al.
Journal of immunology (Baltimore, Md. : 1950), 167(3), 1787-1794 (2001-07-24)
The expression of MHC class II molecules is essential for all Ag-dependent immune functions and is regulated at the transcriptional level. Four trans-acting proteins control the coordinate expression of MHC class II molecules: class II trans-activator (CIITA), regulatory factor binding
Rodrigo Naves et al.
International immunology, 14(5), 481-491 (2002-04-30)
MHC class II expression defects have been evidenced in several human tumor cell lines originating from lung cancers or retinoblastoma. Accordingly, the mouse adenocarcinoma and fibrosarcoma cell lines, RAG and L(tk-), do not express I-A and I-E molecules even when
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