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Safety Information

AB3096

Sigma-Aldrich

Anti-Orexin Antibody, Prepro

Chemicon®, from rabbit

Synonym(s):

Anti-NRCLP1, Anti-OX, Anti-PPOX

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_001524.1

UniProt accession no.

O43612

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PPOX(5498)

Specificity

Mouse Prepro-Orexin. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

Immunogen

A 17 mer peptide (designated PPOXA11-A) mapping near the C-terminus of mouse prepro-Orexin.

Application

Anti-Orexin Antibody, Prepro is an antibody against Orexin for use in ELISA, IH & WB.
Research Category
Neuroscience
Research Sub Category
CNS Control of Metabolism

Circadian Rhythm & Sleep
Western blot: 1-10 μg/mL using Chemiluminescence technique

ELISA: 1:10,000 - 100,000

Immunohistochemistry: 2-20 μg/mL.

Optimal working dilutions must be determined by end user.

Physical form

Liquid in PBS with 0.1% BSA.

Storage and Stability

Can be refrigerated at 2-8°C in undiluted aliquots for short term use. Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB3096:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Exaggerated natriuresis during clamping of systemic NO supply in healthy young men.
Simonsen, JA; Rasmussen, MS; Vach, W; H?ilund-Carlsen, PF; Bie, P
Clinical Science (London, England : 1979) (1979)
Simona Tafuri et al.
Neuropeptides, 43(2), 73-80 (2009-03-03)
The hypothalamic peptide orexin A, deriving from the proteolytic cleavage of the precursor molecule prepro-orexin, has a wide range of physiological effects including the regulation of feeding behaviour, neuroendocrine functions, sleep-wake cycle, and energy homeostasis. Lowered excretion of orexin A
Disorganized epithelial polarity and excess trophectoderm cell fate in preimplantation embryos lacking E-cadherin.
Stephenson RO, Yamanaka Y, Rossant J
Development null
Differential c-Fos immunoreactivity in arousal-promoting cell groups following systemic administration of caffeine in rats.
Samuel Deurveilher, Henry Lo, Jeremy A Murphy, Joan Burns, Kazue Semba
The Journal of Comparative Neurology null
Luigi Michele Pavone et al.
Anatomical record (Hoboken, N.J. : 2007), 292(2), 202-206 (2008-12-18)
The hypothalamic peptide orexin A (oxA) binds specifically the G-protein-coupled orexin receptor 1 (ox1R). It is involved in many physiological functions including the regulation of food intake, sleep-wake cycle, arterial blood pressure, heart rate, and sexual behavior. The localization of
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