524545

Phosphatase, Alkaline, E. coli

• Synonym(s): Phosphatase, Alkaline, E. coli
• CAS Number: 9001-78-9
• Enzyme Commission number: 3.1.3.1 (BRENDA, IUBMB)
• MDL number: MFCD00131849
• UNSPSC Code: 12352202

Properties

• form: liquid
• Quality Level: 100
• specific activity: ≥500 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• pI: 4.5
• foreign activity: Non-specific endonuclease, RNase, none detected
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Native alkaline phosphatase from E. coli. A dimeric zinc- and magnesium-containing protein. A non-specific phosphomonoesterase with a pI of 7.5.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of pNPP per min at 25°C in Tris-HCl, pH 8.0.

Physical form

In 50 mM Tris-HCl, 50% glycerol, pH 7.5.

Other Notes

Reid, T.W. and Willson, I.B. 1971. Enzymes4, 383.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1