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Safety Information

P0114

Sigma-Aldrich

Phosphatase, Alkaline from bovine intestinal mucosa

BioUltra, ≥5,700 DEA units/mg protein

Synonym(s):

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS Number:
9001-78-9
Enzyme Commission number:
3.1.3.1 (BRENDA, IUBMB)
MDL number:
MFCD00131849
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.54

product line

BioUltra

Quality Level

200

form

(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

specific activity

≥5,700 DEA units/mg protein

mol wt

dimer ~160 kDa

concentration

≥10 mg/mL

shipped in

wet ice

storage temp.

2-8°C

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General description

Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
High specific activity grade recommended for antibody and protein conjugation.
Phosphatase alkaline from bovine intestinal mucosa has been used in a study to measure the hydrolysis kinetics of three polyfluorinated alkyl phosphate monoesters, differing in fluorinated chain length. It has also been used in a study to show that the developmentally controlled expression of the isosomes C-1 and C-2 indicate functional changes in intestinal alkaline phosphatase with tissue maturation.

Biochem/physiol Actions

Alkaline phosphatase was shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A.
The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical properties

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Unit Definition

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

Physical form

Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6

Analysis Note

Package sizes are based on DEA units

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P0114-BULK:
P0114-50KU:
P0114-10KU:
P0114-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Suzanne Kats et al.
Recent patents on inflammation & allergy drug discovery, 3(3), 214-220 (2009-06-19)
Laboratory and clinical data have implicated endotoxin as an important factor in the inflammatory response to cardiopulmonary bypass. Alkaline phosphatase prevents endotoxin-induced systemic inflammation in animals and humans. We assessed the effects of the administration of bovine intestinal alkaline phosphatase
Fuhong Su et al.
Critical care medicine, 34(8), 2182-2187 (2006-06-16)
Alkaline phosphatase may decrease the harmful effects of lipopolysaccharide by detoxifying lipid A. The aim of this study was to investigate whether administration of alkaline phosphatase is beneficial in a clinically relevant septic shock model. Interventional laboratory study. University hospital
M Besman et al.
The Journal of biological chemistry, 260(20), 11190-11193 (1985-09-15)
Alkaline phosphatases from calf and bovine small intestines have been isolated in homogeneous form from both mucosa and luminal contents. The detergent-solubilized calf enzyme resolves into two peaks of activity, C-1 and C-2, on chromatofocusing. Only one of these activity
Khanh T T Nguyen et al.
Pharmaceutics, 14(10) (2022-10-28)
Powder bed printing is a 3D-printing process that creates freeform geometries from powders, with increasing traction for personalized medicine potential. Little is known about its applications for biopharmaceuticals. In this study, the production of tablets containing alkaline phosphatase using powder
Zhongxin Zhu et al.
Electrophoresis, 36(6), 924-929 (2014-12-30)
A fluorescent quenching detection method for phosphoproteins in SDS-PAGE by using calconcarboxylic acid (CCA) was described. In this method, the fluorescence intensity of CCA was greatly increased with the presence of Al(3+) in the gel background, while in zones where
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Protocols

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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