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407241

Sigma-Aldrich

Insulin Degrading Enzyme, His•Tag®, Rat, Recombinant, S. frugiperda

Synonym(s):

Insulysin, IDE

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About This Item

Enzyme Commission number:
3.4.24.56 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.25

Assay

≥90% (SDS-PAGE)

Quality Level

100

form

liquid

specific activity

≥3 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−70°C

General description

Recombinant, rat insulin degrading enzyme fused to a His•Tag sequence and expressed in S. frugiperda insect cells. A metalloprotease that degrades insulin and a variety of other peptides including amyloid peptides.
Recombinant, rat insulin degrading enzyme fused to a His•Tag sequence and expressed in S. frugiperda insect cells. Insulin degrading enzyme (Insulysin) is a metalloprotease that degrades insulin and a variety of other peptides, including glucagon, insulin-like growth factors I and II, atrial natriuretic peptide, TGF-α, and amyloid peptides. Reported to be inversely correlated with brain Aβ peptide levels in insulysin-deficient mice compared to wild-type mice.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of the enzyme that will catalyze the hydrolysis of 1 µmol iodinated insulin per h at 37°C, pH 7.3.

Physical form

In 100 mM potassium phosphate buffer, 20% glycerol, pH 7.3.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Hersh, L.B. 2003. Curr. Pharm. Des.9, 449.
Miller, B.C., et al. 2003. Proc. Natl. Acad. Sci. USA100, 6221.
Song, E.S., et al. 2003. J. Biol. Chem.278, 49789.
Mukherjee, A., and Hersh, L.B. 2002. J. Alzheimer′s Dis.4, 341.
Song, E.S., et al. 2001. J. Biol. Chem.276, 1152.
Becker, A.B., and Roth, R.A. 1995. Methods Enzymol.248, 693.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

407241-50UG:
407241-UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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