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Safety Information

235400

Caspase-3 Substrate I, Colorimetric

Colorimetric substrate for caspase-3 (Km = 9.7 µM) and related cysteine proteases.

Synonym(s):

Caspase-3 Substrate I, Colorimetric, Ac-DEVD- pNA, Ac-DEVD-pNA

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About This Item

Empirical Formula (Hill Notation):
C26H34N6O13
CAS Number:
1177131-27-9
Molecular Weight:
638.58
UNSPSC Code:
12352200

Assay

≥95% (HPLC)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

solubility

DMSO: 60 mg/mL

shipped in

ambient

storage temp.

−20°C

General description

Colorimetric substrate for CPP32/Apopain/Yama (Km = 9.7 µM) and related cysteine proteases. Sequence is based on the P1-P4 tetrapeptide cleavage site of poly (ADP-ribose) polymerase (PARP) and includes Asp216. CPP32 is the protease responsible for the cleavage of PARP. Can be used with Pro-caspase-3 in in vitro assays to measure caspase-9 activity, since caspase-9 activates pro-caspase-3. Cleavage of the substrate can be monitored at 405 nm (ε = 9160 cm-1M-1).
Colorimetric substrate for caspase-3 (Km = 9.7 µM) and related cysteine proteases. Sequence is based on the P1-P4 tetrapeptide cleavage site of poly(ADP-ribose) polymerase (PARP) and includes Asp216. Caspase-3 is the protease responsible for the cleavage of PARP. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10. Cleavage of pNA is monitored colorimetrically at ~405 nm.

Biochem/physiol Actions

Cell permeable: no
Km = 9.7 µM as colorimetric substrate for caspase-3
Primary Target
Caspas-3
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Asp-Glu-Val-Asp-pNA

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Cardone, M.H., et al. 1998. Science 282, 1318.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature376, 37.
Lazebnik, Y.A., et al. 1994. Nature371, 346.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

235400-5MG:
235400-MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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