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Safety Information

A2559

Sigma-Aldrich

N-Acetyl-Asp-Glu-Val-Asp p-nitroanilide

≥97% (HPLC), powder

Synonym(s):

Ac-DEVD-pNA

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About This Item

Empirical Formula (Hill Notation):
C26H34N6O13
CAS Number:
189950-66-1
Molecular Weight:
638.58
MDL number:
MFCD00792707
UNSPSC Code:
12352209
PubChem Substance ID:
24890668
NACRES:
NA.32

Quality Level

100

Assay

≥97% (HPLC)

form

powder

color

white

storage temp.

−20°C

SMILES string

CC(C)[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(C)=O)C(=O)N[C@@H](CC(O)=O)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C26H34N6O13/c1-12(2)22(26(43)30-18(11-21(38)39)24(41)28-14-4-6-15(7-5-14)32(44)45)31-23(40)16(8-9-19(34)35)29-25(42)17(10-20(36)37)27-13(3)33/h4-7,12,16-18,22H,8-11H2,1-3H3,(H,27,33)(H,28,41)(H,29,42)(H,30,43)(H,31,40)(H,34,35)(H,36,37)(H,38,39)/t16-,17-,18-,22-/m0/s1

InChI key

GGXRLUDNGFFUKI-ORGXJRBJSA-N

Gene Information

human ... CASP3(836)

Amino Acid Sequence

NAc-Asp-Glu-Val-Asp-pNA

Application

N-Acetyl-Asp-Glu-Val-Asp p-nitroanilide has been used as a chromogenic substrates for determining caspase-3 and caspase-8 activities in liver tissue homogenates and caspase 3 activity in aortic vascular smooth muscle cells and neuroblastoma cell lines.

Biochem/physiol Actions

Colorimetric substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase.

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Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A2559-VAR:
A2559-BULK:
A2559-1MG:
A2559-5MG:
A2559-15MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ouyang Chen et al.
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The study investigated the role of Akt1 through the cardioprotection of high-concentration hydrogen (HCH). C57BL/6 mice were randomly divided into the following groups: sham, I/R, I/R + HCH, I/R + HCH + LY294002 (PI3K inhibitor), I/R + HCH + wortmannin (PI3K inhibitor), I/R + LY294002, and I/R + wortmannin. After 45 min of ischemia, HCH
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