228250

Cholesterol Oxidase, Streptomyces sp.

Cholesterol Oxidase, Streptomyces sp. CAS 9028-76-6, is a native cholesterol oxidase that catalyzes the oxidation of cholesterol to cholesterone and hydrogen peroxide.

• Synonym(s): Cholesterol Oxidase, Streptomyces sp., Cholesterol:oxygen oxidoreductase
• CAS Number: 9028-76-6
• Enzyme Commission number: 1.1.3.6 (BRENDA, IUBMB)
• MDL number: MFCD00130783
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• Quality Level: 100
• form: lyophilized solid
• specific activity: ≥3 units/mg solid; ≥40 U/mg
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: yellow
• solubility: water: 20 mg/mL
• foreign activity: Catalase ≤1%; glucose oxidase and uricase ≤0.01%
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native cholesterol oxidase from Streptomyces species. Catalyzes oxidation of cholesterol to cholesterone and hydrogen peroxide (isoelectric points: 6.1 and 7.3). Suitable for determination of total cholesterol when coupled with cholinesterase and peroxidase. Has an optimal pH of 6.0-8.0.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will oxidize 1.0 µmol of cholesterol per min at 25°C, pH 7.0.

Other Notes

Gadda, G., et al. 1997. Eur. J. Biochem. 250, 369.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable