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Safety Information

2114-M

Millipore

Detergent-OUT Micro Kit

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About This Item

UNSPSC Code:
41116012
eCl@ss:
32160405
NACRES:
NB.24

technique(s)

protein clean-up: suitable

application(s)

sample preparation

General description

The Chemicon Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the Detergent-OUT Spin column and collect detergent free protein solution. Micro columns are suitable for removing detergent from a maximum of 0.2 mL sample volume.

Application

PROTOCOL:

1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.

2. Buffer Equilibration: SpinOUT columns are supplied in deionized water. Equilibrate the column with a buffer of your choice, as follows. Note: Do not equilibrate the columns with Tris buffer. Apply about 0.5 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.

3. Place the column in a 2.0 mL micro-centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.

4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 1-2 minutes. Note: If your protein solution is in Tris buffer, exchange the protein solution buffer to phosphate or other buffer, using either a SpinOUT column or by dialysis.

5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.

Packaging

10 columns

Components

Detergent-OUT Columns-DTG-100X 10 Micro Collection Tubes (2 mL) 10

Storage and Stability

Store at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Poisonous substance

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

2114-M:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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