Skip to Content
Merck
All Photos(4)

Documents

Safety Information

1.40011

Millipore

modified Tryptic Soy Broth- Dehydrated Culture Media

GranuCult® prime, according to USDA-FSIS, granular, suitable for microbiology, pathogen testing

Synonym(s):

GranuCult® modified Tryptic Soy Broth, mTSB, soy broth, CASO Broth, Casein-peptone Soymeal-peptone Broth

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

Agency

according to USDA-FSIS

Quality Level

200

form

granular

manufacturer/tradename

GranuCult® prime

application(s)

food and beverages
microbiology
pathogen testing

suitability

Escherichia coli O157:H7
Escherichia coli (non-O157 Shiga Toxin-Producing (STEC))
Salmonella spp.

Looking for similar products? Visit Product Comparison Guide

General description

mTSB (modified Tryptic Soy Broth) without the addition of novobiocin is a selective enrichment media for the isolation and growth of non-O157 Shiga Toxin-Producing Escherichia coli (STEC) and Salmonella from meat products, carcass, and environmental sponges. For the selective enrichment of Escherichia coli O157:H7 in outbreak-related samples, e.g. fermented sausage, cooked meat products, the addition of novobiocin is recommended. Pancreatic digest of casein, papaic digest of soybean meal, and casamino acids (casein acid hydrolysate) serve as carbon and nitrogen sources, vitamins, and minerals. Glucose also serves as a carbon energy source for rapid growth during the enrichment. Sodium chloride maintains the osmotic balance and dipotassium hydrogen phosphate acts as a buffering agent for maintaining the pH balance of the medium. The addition of Bile salts ensures selectivity of the media.

Application

modified Tryptic Soy Broth has emerged as the medium of choice for the enrichment of E. coli and Salmonella species in red meats. This medium is not intended for use in the diagnosis of a disease or other conditions in humans.

Features and Benefits

mTSB media renders the isolation simpler, less costly, and substantially suppresses the growth of other gram-negative Enterobacteriaceae species by 90%.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service