1.11955

Hemacolor^® Rapid staining of blood smear

Solution 1: fixing solution

• Synonym(s): Methanol, MeOH, Hydroxymethane, Methyl alcohol, Carbinol, Methyl alcohol
• Linear Formula: CH₃OH
• CAS Number: 67-56-1
• Molecular Weight: 32.04
• Beilstein: 1098229
• MDL number: MFCD00004595
• EC Index Number: 200-659-6

Properties

• vapor density: 1.11 (vs air)
• Quality Level: 400
• vapor pressure: 128 hPa ( 20 °C); 410 mmHg ( 50 °C); 97.68 mmHg ( 20 °C)
• form: liquid
• autoignition temp.: 725 °F
• potency: 5628 mg/kg LD₅₀, oral (Rat); 17100 mg/kg LD₅₀, skin (Rabbit)
• expl. lim.: 36 %
• IVD: for in vitro diagnostic use
• refractive index: n20/D 1.329 (lit.)
• kinematic viscosity: 0.54-0.59 cSt(20 °C)
• bp: 64.7 °C (lit.)
• mp: −98 °C (lit.)
• transition temp: flash point 9.7 °C
• density: 0.791 g/mL at 25 °C (lit.)
• application(s): clinical testing; diagnostic assay manufacturing; hematology; histology
• storage temp.: 15-25°C
• SMILES string: CO
• InChI: 1S/CH4O/c1-2/h2H,1H3
• InChI key: OKKJLVBELUTLKV-UHFFFAOYSA-N

Description

General description

Hemacolor^® Rapid staining of blood smear - Solution 1: fixing solution, is a ready-to-use solution used for human-medical cell diagnosis and serves the fixing of native blood and bone-marrow smears of human origin in hematological and clinico-cytological specimen materials, for example smears of whole blood and bone marrow. The staining with Hemacolor^® yields a staining result that corresponds to the Pappenheim stain, with predominantly magenta stained nuclei. This is based on the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. By using pH 7.2 buffered solutions with the Hemacolor^® Rapid staining of blood smear solutions, a high stability of the stain and clean, precipitation-free staining results can be guaranteed.
The package is sufficient for 1250 - 5000 applications. The buffer tablets according to Weise pH 7.2 (Product number 1.09468) are also available in larger pack sizes, thus, they can be useful for higher demands. The three solutions needed for staining are all available individually as:

Hemacolor^® Rapid staining of blood smear solution 1, fixing solution (Product number 1.11955)
Hemacolor^® Rapid staining of blood smear solution 2, colour reagent red (Product number 1.11956)
Hemacolor^® Rapid staining of blood smear solution 3, colour reagent blue (Product number 1.11957)

or they can be purchased as staining sets:

Hemacolor^® Rapid staining of blood smear (Product number 1.11674) 3 x 100 ml
Hemacolor^® Rapid staining of blood smear (Product number 1.11661) 3 x 500 ml

For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.

Legal Information

HEMACOLOR is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS02,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H225 - H301 + H311 + H331 - H370
• Precautionary Statements: P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): 49.5 °F - closed cup
• Flash Point(C): 9.7 °C - closed cup