Merck
All Photos(2)

1.00327

Sigma-Aldrich

Hydrochloric acid in ethanol

for microscopy

Synonym(s):
Hydrogen chloride solution, Hydrogen chloride – ethanol solution, Hydrochloric acid in ethanol
Linear Formula:
HCl · CH3CH2OH
CAS Number:
Molecular Weight:
36.46
Beilstein:
1098214
MDL number:

Quality Level

form

liquid

IVD

for in vitro diagnostic use

pH

1.0 (20 °C in H2O)

transition temp

flash point 21 °C

density

0.90 g/cm3 at 20 °C

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

SMILES string

Cl

InChI

1S/ClH/h1H

InChI key

VEXZGXHMUGYJMC-UHFFFAOYSA-N

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General description

Hydrochloric acid in ethanol - for microscopy, is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. With the Kinyoun method, a modification of the AFB-Color carbol fuchsin solution renders heating unnecessary (cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Product number 1.00327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
Pretreatment of the specimens with Sputofluol dissolves the bacteria from the surrounding viscid sputum and cell material. It is an IVD and CE registered product and can be applied for diagnostical purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

For microscopy. Ziehl-Neelsen staining

Legal Information

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

Pictograms

FlameCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

69.8 °F

Flash Point(C)

21 °C

Certificate of Analysis

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Certificate of Quality

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