07-645
Anti-Sp1 Antibody
Upstate®, from rabbit
Synonym(s):
Sp1 transcription factor, specificity protein 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, rat
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
electrophoretic mobility shift assay: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... SP1(6667)
General description
Sp1 is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 95 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.
Specificity
Expected to cross-react with rat.
Recognizes Sp1, Mr 105 kDa and 95 kDa.
Immunogen
Full length human Sp1 protein.
Application
Immunoprecipitation:
4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). See manual for more information and protocol: www.millipore.com
Chromatin Immunoprecipitation:
Reported by an independent laboratory.
Immunofluorescence:
Reported by an independent laboratory.
Electrophoretic Mobility Shift Assay (EMSA):
1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.
4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). See manual for more information and protocol: www.millipore.com
Chromatin Immunoprecipitation:
Reported by an independent laboratory.
Immunofluorescence:
Reported by an independent laboratory.
Electrophoretic Mobility Shift Assay (EMSA):
1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-Sp1 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, EMSA, IF, IP, WB to detect Sp1 also known as Sp1 transcription factor, specificity protein 1.
Quality
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis:
1:500 dilution of this lot detected SP1 on 10 μg of HeLa lysates.
Western Blot Analysis:
1:500 dilution of this lot detected SP1 on 10 μg of HeLa lysates.
Target description
Varies
Linkage
Replaces: MABE550
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Heme oxygenase-1/carbon monoxide induces vascular endothelial growth factor expression via p38 kinase-dependent activation of Sp1.
Lin, HH; Lai, SC; Chau, LY
The Journal of Biological Chemistry null
Sheau-Chung Tang et al.
Cancer, 117(14), 3209-3221 (2011-01-20)
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Constitutive expression of the brg1 gene requires GC-boxes near to the transcriptional start site.
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DHT selectively reverses Smad3-mediated/TGF-beta-induced responses through transcriptional down-regulation of Smad3 in prostate epithelial cells.
Song, K; Wang, H; Krebs, TL; Wang, B; Kelley, TJ; Danielpour, D
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