07-1820

Anti-EEA1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): early endosome antigen 1, early endosome-associated protein, early endosome antigen 1, 162kD, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μL
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_003557
• UniProt accession no.: Q15075
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... EEA1(8411)

Description

General description

Early endosome antigen 1 (EEA1) is a 170 kDa coiled-coil protein belonging to a group of early endosomal Rab5 effector proteins. EEA1 has a putative role in the tethering/docking of endocytic vesicles prior to their fusion with early endosomes. This protein is characterized as containing a zinc finger-like domain and coiled-coil regions. The zinc finger-like domain, also known as the FYVE finger, is a motif rich in cystine, is a necessary component of EEA1’s ability for endosomal targeting, and is specifically known for its dual interaction with Rab5 GTPase and phosphotidylinositol 3-phosphate (Ptdlns-3-P).

Specificity

Equine and Canine (99% sequence homology). Rat (98% sequence homology). Bovine (97% sequence homology).

This antibody recognizes EEA1.

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human EEA1.

Application

Research Category
Signaling

Research Sub Category
General Post-translation Modification

This Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1.

Western Blot (SNAP ID) Analysis: 1:250 dilution from a previous lot detected EEA1 on 10 µg of C2C12 cell lysate.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells.

Quality

Evaluated by Western Blot in BALB 3T3/A31 cell lysate.

Western Blot Analysis: A 1:1000 dilution of this antibody detected EEA1 on 10 µg of BALB 3T3/A31 cell lysate.

Target description

~ 170 kDa

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
BALB 3T3/A31 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable