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Key Documents

Safety Information

05-776

Sigma-Aldrich

Anti-ZAP-70 Antibody, clone 1E7.2

clone 1E7.2, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1E7.2, monoclonal

species reactivity

human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001079

UniProt accession no.

P43403

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Ppp1Ca(19045)

General description

Note: This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California

Specificity

Zap-70

Immunogen

GST fusion protein between the second SH2 domain and precedes the kinase domain corresponding to residues 282-307 of human ZAP-70

Application

Anti-ZAP-70 Antibody, clone 1E7.2 is an antibody against ZAP-70 for use in IC, IP & WB.
Research Category
Signaling
Research Sub Category
Immunological Signaling

Quality

routinely evaluated by immunoblot on RIPA lysates from Jurkat cells

Target description

Mr ~70kDa

Physical form

Format: Purified
Protein G Purified
of 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

Other Notes

Due to license agreement restrictions, this product cannot be purchased for resale.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05-776:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kristin Hochweller et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(13), 5931-5936 (2010-03-17)
Dendritic cells (DCs) are key components of the adaptive immune system contributing to initiation and regulation of T cell responses. T cells continuously scan DCs in lymphoid organs for the presence of foreign antigen. However, little is known about the
TCR signaling: another Abl-bodied kinase joins the cascade.
Wange, Ronald L
Current Biology, 14, R562-R564 (2004)

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