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Safety Information

05-1588

Sigma-Aldrich

Anti-U1-70K Antibody, clone 9C4.1

clone 9C4.1, from mouse

Synonym(s):

small nuclear ribonucleoprotein 70kDa (U1), U1 small nuclear ribonucleoprotein 70 kDa, small nuclear ribonucleoprotein 70kDa (RNP antigen), U1 snRNP 70 kDa

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

9C4.1, monoclonal

species reactivity

rat, mouse, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_003080.2

UniProt accession no.

P08621

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SNRNP70(6625)

General description

U1 small nuclear ribonucleoprotein 70 kDa (U1-70K) is one of three specific proteins found within the U1 snRNP core, which includes at least seven core proteins found in all U snRNPs and three specific proteins; U1-70K, U1-A, and U1-C. U1-70K is involved in mediating pre-mRNA splicing through binding the loop I region of U1-snRNA with its RPM (RNA recognition motif) binding domain. In addition, its RNA binding domain, U1-70K also contains two argine-rich regions, accented with acidic or serine residues, that is thought to have trans-acting functions. Alternative splicing results in 4 known isoforms. Truncated forms of U1-70K are unable to bind RNA.

Specificity

This antibody recognizes U1-70K at the C-terminal domain.

Immunogen

Epitope: C-terminal domain
Recombinant protein corresponding to C-terminal domain of human U1-70K.

Application

Immunocytochemistry Analysis: A 1:500 dilution of a representative lot of this antibody has been shown to detect U1-70K in NIH/3T3, A431, and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Use Anti-U1-70K Antibody, clone 9C4.1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect U1-70K also known as small nuclear ribonucleoprotein 70kDa (U1).

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected U1-70K on 10 µg of HeLa cell lysate.

Target description

Calculated 52 kDa Observed ~57 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05-1588:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The Journal of Clinical Investigation null
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Nucleic acids research, 47(8), 4181-4197 (2019-02-16)
Src associated in mitosis (SAM68) plays major roles in regulating RNA processing events, such as alternative splicing and mRNA translation, implicated in several developmental processes. It was previously shown that SAM68 regulates the alternative splicing of the mechanistic target of
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The dynamic and reversible N6-methyladenosine (m6A) RNA modification installed and erased by N6-methyltransferases and demethylases regulates gene expression and cell fate. We show that the m6A demethylase ALKBH5 is highly expressed in glioblastoma stem-like cells (GSCs). Silencing ALKBH5 suppresses the

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