R3380
Ribonucleoside vanadyl complexes
200 mM
Synonym(s):
vanadyl ribonucleoside complexes
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General description
Ribonucleoside vanadyl complexes are stable complexes formed between ribonucleotides. They are low molecular weight inhibitors of cellular ribonucleases (RNases) that are used for the isolation of RNA from cells.
Application
Ribonucleoside vanadyl complexes are used as ribonuclease inhibitors during cell lysis and mRNA purification, to protect mRNA during cDNA production, and to protect RNA during digestion of DNA by DNaseI.
Ribonucleoside vanadyl complexes has been used:
- as a component of lysis buffer for cell lines prior to Poly(U) pull down assay
- to inhibit nucleases prior to immunoprecipitation (IP) of m6A-containing RNA fragment
- as a component of hybridization buffer for human retinal slices
Biochem/physiol Actions
Ribonucleoside vanadyl complexes (RVC) elicits antimicrobial functionality by inhibiting ribosomal subunit formation and suppressing the growth of Staphylococcus aureus and Escherichia coli. It is also used as a preservative solution for tissues.
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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DNA damage induces p21 protein expression by inhibiting ubiquitination in ML-1 cells.
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