L021000

DCA Deblock

with Dichloromethane

• UNSPSC Code: 12352200

Properties

• Quality Level: 200
• type: for DNA synthesis; for DNA synthesis
• product line: Proligo Reagents
• form: liquid
• storage condition: dry at room temperature
• concentration: 42.2-51.6 g/L (DCA content, titration)
• impurities: ≤200 ppm water content (Karl Fischer)
• color: clear

Description

General description

Deblock solution contains dichloroacetic acid (DCA) in dichloromethane that assists in the removal of the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, before the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Preparation Note

Dicloromethane / Dichloroacetic acid 97/3 (v/v)

Other Notes

Deblock solution containing dichloroacetic acid (DCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Safety Information

• Pictograms: GHS05,GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H315 - H318 - H336 - H351 - H360FD - H362
• Precautionary Statements: P202 - P263 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Carc. 2 - Eye Dam. 1 - Lact. - Repr. 1B - Skin Irrit. 2 - STOT SE 3
• Target Organs: Central nervous system
• Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3