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81240

Sigma-Aldrich

Poly(ethylene glycol)

average Mn 4,000, hydroxyl

Synonym(s):

Polyethylene glycol, PEG

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About This Item

Linear Formula:
H(OCH2CH2)nOH
CAS Number:
25322-68-3
MDL number:
MFCD00081839
UNSPSC Code:
12352104
PubChem Substance ID:
24887706
NACRES:
NA.23

product name

Poly(ethylene glycol), average Mn 4,000, platelets

biological source

platelets

Quality Level

200

form

powder

mol wt

Mn 3,500-4,500
average Mn 4,000

mp

58-61 °C

Ω-end

hydroxyl

α-end

hydroxyl

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

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General description

Polyethylene glycol (PEG) is a hydrophilic polymer. It can be easily synthesized by the anionic ring opening polymerization of ethylene oxide, into a range molecular weights and variety of end groups. When crosslinked into networks PEG can have high water content, forming “hydrogels PEG is a suitable material for biological applications because it does not trigger an immune response.

Application

Polyethylene glycol (PEG) platelets were used:
  • in PEG-directed gene delivery of protoplast transfection and incubation
  • to prepare extraction for measuring cytosolic phosphoenolpyruvate carboxykinase (PEPCK) from plant tissues
  • as DNA transformation agent in a study of transient gene expression of plant protoplasts, derived from rice leaf sheaths.
  • in preparing isothermal assembly buffer and TSS broth preparation for unique nucleotide sequence (UNS)-guided assembly of repetitive DNA parts
  • in transformation of DNA into log-phase cells of the budding yeast Saccharomyces cerevisiae.
  • as DNA transformation reagent

Other Notes

Polymer networks with grafted cell adhesion peptides for highly biospecific cell adhesive substrates

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

81240-5KG:
81240-VAR:
81240-BULK:
81240-6X1KG:
81240-1KG:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG5818
BCCG5820
BCCF2031
BCCD2479
BCCD2478

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Oleg Raitskin et al.
PloS one, 14(2), e0211598-e0211598 (2019-02-28)
Molecular tools adapted from bacterial CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats) systems for adaptive immunity have become widely used for plant genome engineering, both to investigate gene functions and to engineer desirable traits. A number of different Cas (CRISPR-associated)
Shen, Jinbo, et al. "Isolation, culture, and transient transformation of plant protoplasts
Shen J, et al.
Current Protocols in Cell Biology, 2014, 2-8 null
A simple and effective method to encapsulate tobacco mesophyll protoplasts to maintain cell viability
Lei R, et al.
Methods, 24-32 (2015)
Jennifer DeMars Tripp et al.
Yeast (Chichester, England), 30(5), 191-200 (2013-03-14)
Chemical-based methods have been developed for transformation of DNA into log-phase cells of the budding yeast Saccharomyces cerevisiae with high efficiency. Transformation of early stationary-phase cells, e.g. cells grown in overnight liquid cultures or as colonies on plates, is less
Frédéric Sanchez et al.
Genes, 10(5) (2019-05-28)
Ostreococcustauri is an easily cultured representative of unicellular algae (class Mamiellophyceae) that abound in oceans worldwide. Eight complete 13-22 Mb genomes of phylogenetically divergent species within this class are available, and their DNA sequences are nearly always present in metagenomic
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