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Safety Information

227935

Sigma-Aldrich

Azure B

prepared by direct synthesis

Synonym(s):

N,N,N′-Trimethylthionin, Azure I, Methyleneazure

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About This Item

Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
531-55-5
Molecular Weight:
305.83
Colour Index Number:
52010
Beilstein:
3922630
EC Number:
208-511-2
MDL number:
MFCD00011935
UNSPSC Code:
12171500
PubChem Substance ID:
24853664
NACRES:
NA.47

Assay

>85.0% (HPLC)

form

powder

mp

205-210 °C (dec.) (lit.)

solubility

water: 1 mg/mL, blue to very deep blue

λmax

648 nm

ε (extinction coefficient)

≥13000 at 241-247 nm
≥36000 at 287-293 nm
≥67000 at 638-644 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]

InChI

1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H

InChI key

DNDJEIWCTMMZBX-UHFFFAOYSA-N

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Application

Azure B has been used in M′Fadyean stain to visualize Bacillus anthracis capsule.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localization of nucleic acids and lignin.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

227935-5G:
227935-100G:
227935-BULK:
227935-H:
227935-1KG:
227935-VAR:
227935-25G:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Barbara J. Bain, Imelda Bates, Mike A Laffan
Dacie and Lewis Practical Haematology (2016)
A simple, reliable M'Fadyean stain for visualizing the Bacillus anthracis capsule
Owen MP
Journal of Microbiological Methods, 92, 264-269 (2013)
U.S. Forest Service research note FPL (1964)
Anél Petzer et al.
Toxicology and applied pharmacology, 258(3), 403-409 (2011-12-27)
Methylene blue (MB) has been shown to act at multiple cellular and molecular targets and as a result possesses diverse medical applications. Among these is a high potency reversible inhibition of monoamine oxidase A (MAO-A) that may, at least in
S Rodríguez Couto et al.
Chemosphere, 46(1), 83-86 (2002-01-25)
This work reports a preliminary design of a new photochemical reactor and its application to photochemical degradation of two dyes, Crystal Violet and Azure B, operating in both batch and continuous processes. A novel kind of photocatalyst, consisting of ZnO
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