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Key Documents

Safety Information

66720

Sigma-Aldrich

Methylene Blue hydrate

purum p.a., for microscopy (Bact., Bot., Hist., Vit.), ≥97.0% (calc. based on dry substance, AT)

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
Molecular Weight:
319.85 (anhydrous basis)
Beilstein:
3599847
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32

grade

for microscopy (Bact., Bot., Hist., Vit.)
purum p.a.

Quality Level

Assay

≥97.0% (calc. based on dry substance, AT)

SMILES string

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

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General description

Methylene Blue (MB) is a basic histological dye.

Application

Methylene Blue hydrate has been used:
  • in the preparation of methylene blue (MB) solution to stain zymograms
  • to label the injection site in the tissue slices
  • to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss

Biochem/physiol Actions

Methylene blue (MB) plays a key role in microbiology and pharmacology. It is mainly used in staining live organisms and in methemoglobinemia treatment. MB can be considered as a possible and safe vasoconstrictor. It has several applications in textile, paper and hair coloring sectors.

Other Notes

may contain up to 22% water
On the efficiency of methylene blue versus persulfate catalysis of polyacrylamide gels, as investigated by capillary zone electrophoresis

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

66720-VAR:
66720-100G:
66720-BULK:
66720-500G:
66720-25G:


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UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1-/- Microglia In Vitro and In Vivo
Lim HM, et al.
Mediators of Inflammation, 2017(3), e1687-e1687 (2017)
Efficient removal of different basic dyes using graphene
Iqbala MZ, et al.
Desalination and Water Treatment, 68(3), 226-235 (2017)
Preparation and analysis of crude autolytic enzyme extracts from Staphylococcus aureus
Vaz F, et al.
Bio-protocol, 5(3), e1687-e1687 (2015)
Attenuation of noise-induced hearing loss using methylene blue
Park JS, et al.
Cell Death & Disease, 5(4), e1200-e1200 (2014)
S Caglio et al.
Electrophoresis, 14(10), 997-1003 (1993-10-01)
The efficiency of a novel method of photopolymerization, based on photoinitiating the reaction with methylene blue (MB), in presence of a redox couple (sodium toluenesulfinate and diphenyliodonium chloride), vs, the conventional persulfate--N,N,N',N'-tetramethylethylenediamine redox couple was investigated as a function of

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