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HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis® Express C18 after Phospholipid Removal using HybridSPE®-Phospholipid

HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis® Express C18 after Phospholipid Removal using HybridSPE®-Phospholipid application for SPE, application for LC-MS

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

53822-U

Colonna per HPLC Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

Colonnina o piastra per SPE

N° Catalogo
Descrizione
Determinazione del prezzo

575656-U

HybridSPE®-Phospholipid

96-well Plate, bed wt. 50 mg, volume 2 mL, pk of 1

CONDITIONS

SPE well plate

HybridSPE-Precipitation 96-well Plate, 50 mg/well (575656-U)

sample preparation

SPE (Solid Phase Extraction)

column

Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)

sample/matrix

Rat plasma spiked with verapamil and metabolites at 10 ng/mL

mobile phase

[A] water; [B] acetonitrile, pH 2.55 adjust with formic acid (30:70, A:B)

flow rate

0.6 mL/min

column temp.

35 °C

detector

TOF/MS

injection

1 μL

sample addition

100 μL spiked rat plasma followed by 300 μL 1% formic acid in acetonitrile

elution

apply vacuum

Descrizione

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS