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HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 5:95)

HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 5:95) application for HPLC

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

53981-U

Ascentis® Express HILIC HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Standard

N° Catalogo
Descrizione
Determinazione del prezzo

C7252

D-(+)-cellobiosio

≥98% (HPLC)

A3256

L-(+)-arabinosio

≥99% (GC)

84097

Saccarosio

BioUltra, ≥99.5% (HPLC)

1287700

Galattosio

United States Pharmacopeia (USP) Reference Standard

G0350500

D-(+)-Glucosio

European Pharmacopoeia (EP) Reference Standard

X0200000

Xylose

European Pharmacopoeia (EP) Reference Standard

1375182

D-(+)-mannosio

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] water; [B] acetonitrile; (5:95, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

Descrizione

Risultati analitici

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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