HPLC Analysis of Ephedrine Enantiomers on CHIRALPAK^® AGP

column

CHIRAL-AGP, 10 cm x 4 mm I.D., 5 μm particles (58170AST)

mobile phase

1 mM octanoic acid in 10 mM (NH₄)₂HPO₄, pH 6.9

flow rate

0.9 mL/min

column temp.

25 °C

detector

UV, 225 nm

injection

10 μL

sample

1 mg/mL in methanol