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58295C40

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SUPELCOSIL™ LC-Si (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

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About This Item

Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

SUPELCOSIL™ LC-Si HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm

Materiali

stainless steel column

agenzia

suitable for USP L3

Nome Commerciale

SUPELCOSIL™

Caratteristiche

endcapped: no

Produttore/marchio commerciale

SUPELCOSIL™

Confezionamento

1 ea of

Parametri

≤70 °C temp. limit
400 bar pressure (5801 psi)

tecniche

HPLC: suitable

Lungh. × D.I.

25 cm × 4 mm

Area superficiale

170 m2/g

Matrice

silica gel, spherical particle platform
fully porous particle

Gruppo funzionale matrice

silica phase

Dimensione particelle

5 μm

Dimensione pori

120 Å pore size

Intervallo di pH

2-7.5

applicazioni

food and beverages

Tecnica di separazione

hydrophilic interaction (HILIC)
normal phase

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Categorie correlate

Descrizione generale

A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent. Non-polar compounds will elute from the column first, while polar solutes show stronger interaction with the silanol groups on the silica surface. The polar selectivity of silica is very helpful for separating mycotoxins. Silica is frequently used in preparative chromatography due to its low operating cost and back pressure compared to reversed-phase columns. Silica is particularly popular among organic chemists as a low-pressure preparative tool. The unmodified silica in SUPELCOSIL LC-Si columns also separate positional isomers.

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Note legali

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Certificati d'analisi (COA)

Lot/Batch Number
279432-01
279406-01
271689-02
271689-01
271895-02

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J J Agren et al.
Lipids, 37(6), 613-619 (2002-07-18)
Normal-phase HPLC resolution of sn-1,2(2,3)- and x-1,3-DAG generated by partial Grignard degradation from natural TAG was carried out with both (R)-(-) and (S)-(+)-1-(1-naphthyl)ethylurethane derivatives. The diastereomeric sn-1,2- and sn-2,3-DAG derivatives were resolved using two Supelcosil LC-Si (5 microm, 25 cm
Mohammed Shahid Ali et al.
Journal of pharmaceutical and biomedical analysis, 43(1), 158-167 (2006-08-05)
A new HILIC method has been developed for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold syrup. Mobile phase consists of methanol:water (containing 6.0 g of ammonium acetate and 10 mL of
R Lovlin et al.
Journal of chromatography. B, Biomedical applications, 679(1-2), 196-198 (1996-04-26)
The stereospecific HPLC assays reported for ketoprofen (KT) mainly utilize indirect approaches. These assays involve the formation of amide diastereomeric derivatives, which are then separated by chromatography. The advantages of indirect methods include versatility, good sensitivity and cost effectiveness; however

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