Y2377

2x YT medium

Powder microbial growth medium

• Sinonimo/i: 2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media
• Codice UNSPSC: 41106225
• NACRES: NA.85

Proprietà

• Grado: for molecular biology
• Livello qualitativo: 200
• Sterilità: non-sterile
• Stato: powder
• tecniche: microbiological culture: suitable
• pH: 6.8-7.2(3.1% solution)
• applicazioni: food and beverages; microbiology
• Temperatura di conservazione: room temp
• Compatibilità: nonselective for Escherichia coli; nonselective for coliforms

Descrizione

Descrizione generale

2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Applicazioni

2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.

Caratteristiche e vantaggi

2xYT powder provides:

• Standard formulation
• An inexpensive alternative to liquid

Componenti

16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl

Nota sulla preparazione

1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.

Ricostituzione

Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)