TOX7
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
Sinonimo/i:
LDH cytotoxicity assay
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About This Item
Codice UNSPSC:
12352207
NACRES:
NA.84
Prodotti consigliati
Forma fisica
liquid
Livello qualitativo
impiego
kit sufficient for 500 tests
Confezionamento
pkg of 1 kit
Condizioni di stoccaggio
dry at room temperature
tecniche
protein quantification: suitable
λmax
490 nm
applicazioni
cell analysis
detection
Metodo di rivelazione
colorimetric
Temperatura di conservazione
−20°C
Descrizione generale
Tox7 is a member of Sigma′s CytoToxicity Kit series. It is based on the measurement of lactate dehydrogenase (LDH) release from cells as a measure of cell membrane damage and cytotoxicity. This technique has been utilized as an alternative to 51Cr release for cell mediated cytotoxicity assays, as well as conventional cytotoxicity resulting from interaction of a test material with the cell.
Applicazioni
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based has been used:
- to detect lactate dehydrogenase release in human umbilical vein endothelial cells (HUVECs) and human cardiac myocytes (HCMs) for lactate dehydrogenase (LDH) assay
- to determine the activity of lactate dehydrogenase leakage of MDA-MB-231 cells
- to examine the activity of LDH in RAW 264.7 cells , in LDH cytotoxicity assay in human cell lines
- for spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 490 nm.
Azioni biochim/fisiol
The lactate dehydrogenase assay is a means of measuring either the number of cells via total cytoplasmic lactate dehydrogenase (LDH) or membrane integrity as a function of the amount of cytoplasmic LDH released into the medium. This method is simple, accurate and yields reproducible results. The assay is based on the reduction of NAD by LDH. The resulting reduced NAD (NADH) is utilized in the stoichiometric conversion of a tetrazolium dye to a soluble, colored formazan derivative.
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Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3
Codice della classe di stoccaggio
10 - Combustible liquids
Certificati d'analisi (COA)
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Articoli
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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