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T1327

Sigma-Aldrich

Tissue Inhibitor of Metalloproteinase-3

recombinant, expressed in NSO cells, >95% (SDS-PAGE)

Sinonimo/i:

TIMP-3

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About This Item

Numero MDL:
Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

human

Livello qualitativo

Ricombinante

expressed in NSO cells

Saggio

>95% (SDS-PAGE)

Stato

lyophilized powder

PM

apparent mol wt ~30 kDa

tecniche

inhibition assay: suitable

N° accesso UniProt

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... TIMP3(7078)

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Descrizione generale

Tissue inhibitor of metalloproteinases 3 (TIMP3) is localized in the extracellular matrix (ECM) of epithelial cells associated with kidneys, eyes and lungs. It is majorly secreted in retinal pigment epithelium (RPE). TIMP3 gene is mapped to human chromosome 22q12.3 and is a CLOCK-dependent diurnal gene. TIMP proteins display a three-lobed structure and have conserved cysteine residues.

Azioni biochim/fisiol

Tissue inhibitor of metalloproteinases 3 (TIMP3) is a matrix metalloproteinase inhibitor. TIMP proteins comprise the N-terminal region, which aids in the matrix metalloproteinase interaction. The C-terminal region is crucial for extracellular matrix (ECM) interaction. Elevated expression of TIMP3 favors apoptosis in cancer types. Its interaction with the vascular endothelial growth factor (VEGFR-2) leads to the regulation of angiogenesis. TIMP3 also aids in protection against ultra-violet (UV)-induced cellular responses. Missense mutations in the TIMP3 gene are implicated in Sorsby′s fundus dystrophy (SFD). Mutations in the TIMP3 gene also leads to increased accumulation of the protein resulting in the thickening of the Bruch membrane. This, in turn, reduces membrane permeability towards nutrients and metabolites.
The TIMPs are endogenous inhibitors of the matrix metalloproteinases (MMPs). TIMP-3 inhibits ADAM-17 (TACE) at nanomolar concentrations and also inhibits other metalloproteinases (sheddases) that mediate the shedding of soluble receptors and other proteins from the surface of cells.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in 25 mM Tris and 0.15 M sodium chloride, pH 7.5.

Risultati analitici

The biological activity is measured by its ability to inhibit human MMP-2 hydrolysis of a peptide substrate.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves


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Ruth Appeltant et al.
Animal science journal = Nihon chikusan Gakkaiho, 88(9), 1279-1290 (2017-01-27)
In vitro maturation (IVM) in serum causes hampered expansion of porcine cumulus-oocyte complexes (COCs) due to excessive alpha
S M Silbiger et al.
Gene, 141(2), 293-297 (1994-04-20)
Proteins of the tissue inhibitor of metalloproteinase (TIMP) family bind and inactivate matrix metalloproteinases such as collagenases and gelatinases. We report the cloning and sequencing of cDNAs encoding a novel human TIMP, which we designated TIMP-3, the third member of
K J Leco et al.
The Journal of biological chemistry, 269(12), 9352-9360 (1994-03-25)
We have isolated cDNA clones corresponding to a novel mouse metalloproteinase inhibitor. Five overlapping cDNA clones contain most of the information for a prominent 4.5-kilobase transcript that was detected in RNA from mouse fibroblasts and adult tissues. Sequence analysis revealed
A Amour et al.
FEBS letters, 435(1), 39-44 (1998-10-02)
TNF-alpha converting enzyme (TACE; ADAM-17) is a membrane-bound disintegrin metalloproteinase that processes the membrane-associated cytokine proTNF-alpha to a soluble form. Because of its putative involvement in inflammatory diseases, TACE represents a significant target for the design of specific synthetic inhibitors
Han-Li Huang et al.
Theranostics, 9(22), 6676-6689 (2019-10-08)
Tissue inhibitors of metalloproteinase 3 (TIMP3) are a major endogenous inhibitor of matrix metalloproteinase (MMPs) that inhibit tumor growth, invasion, metastasis and angiogenesis. In this study, we found that TIMP3 expression is associated with positive prognosis of colorectal cancer (CRC)

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