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Key Documents

SHM02

Sigma-Aldrich

ExpressMag® 96-Well Magnetic Kit

Increases transduction efficiency

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About This Item

Codice UNSPSC:
41106609
NACRES:
NA.51

Nome Commerciale

MISSION®

Durata

≥2 yr at ~4 °C

Temperatura di conservazione

2-8°C

Descrizione generale

To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols

Applicazioni

MISSION® ExpressMag® 96-Well Magnetic Kit contains all of the components needed to start enhancing your lentiviral transductions. The kit contains a 100 μL vial of ExpressMag™ Beads, enough for 63 wells of a 96 well plate. These beads bind the lentiviral particles and enable the ExpressMag™ magnetic plate to pull the virus down to the surface of cells thereby significantly increasing transduction efficiency. This product is specifically designed to work with 96-well plates. The kit has been documented to enhance MISSION lentiviral transduction of suspension 293T as well as allowing the transduction of primary human cells such as keratinocytes at MOI as low as 1. See the technical bulletin for other cell lines transduced with the ExpressMag system.
MISSION® ExpressMag® 96-Well Magnetic Kit has been used in hepatitis C virus (HCV) replicon luciferase assay.

Attenzione

Magnets should be kept away from magnetic storage devices, such as hard disks, ID cards, and credit cards, and from electronic devices and ferromagnetic materials. Persons with cardiac pacemakers should not work with these magnets.

Note legali

ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS

  • SHM05MISSION® ExpressMag® 96-Well Magnet, Increases transduction efficiencySDS

  • SHM03MISSION® ExpressMag® Beads, Increases transduction efficiencySDS


Certificati d'analisi (COA)

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Knockdown of USP18 increases alpha 2a interferon signaling and induction of interferon-stimulating genes but does not increase antiviral activity in Huh7 cells
Murray EJ, et al.
Antimicrobial Agents and Chemotherapy, 55(9), 4311-4319 (2011)

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