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SHC001V

Sigma-Aldrich

MISSION® pLKO.1-puro Empty Vector Control Transduction Particles

Contains no shRNA insert

Sinonimo/i:

MISSION®, MISSION® Control Transduction Particles

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.51

Livello qualitativo

100
200

Nome Commerciale

MISSION®

Concentrazione

≥1x106 VP/ml (via p24 assay)

tecniche

capture ELISA: 106 TU/mL using p24

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

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Descrizione generale

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Applicazioni

To see more application data, protocols, vector maps visit sigma.com/shrna.

Note legali

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Raccomandato

N° Catalogo
Descrizione
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SHC002

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes

SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC002V

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes

SHC003V

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency

SHC004

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

SHC004V

MISSION® TurboGFP™ shRNA Control Transduction Particles, shRNA sequence targeting tGFP

SHM01

ExpressMag® Super Magnetic Kit, Increases transduction efficiency

SHM02

ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency

SHC016V

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles, Targets no known genes from any species

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Chemoresistance is associated with increased cytoprotective autophagy and diminished apoptosis in bladder cancer cells treated with the BH3 mimetic (-)-Gossypol (AT-101).
Mani J
BMC Cancer, 15, 224-224 (2015)
Soft drug-resistant ovarian cancer cells invade via two distinct mechanisms utilizing myosin IIB
Aastha Kapoor
Nature, 099655-099655 (2017)
Efficacy of adjuvant chemotherapy for non-small cell lung cancer assessed by metastatic potential associated with ACTN4.
Miura N
Oncotarget (2016)
Xiaobo Luo et al.
The Journal of clinical investigation, 130(4), 1635-1652 (2019-12-25)
The incidence of human papillomavirus-positive (HPV+) head and neck squamous cell carcinoma (HNSCC) has surpassed that of cervical cancer and is projected to increase rapidly until 2060. The coevolution of HPV with transforming epithelial cells leads to the shutdown of
JinSeok Park et al.
Nature communications, 10(1), 2797-2797 (2019-06-28)
Collective cell migration occurs in many patho-physiological states, including wound healing and invasive cancer growth. The integrity of the expanding epithelial sheets depends on extracellular cues, including cell-cell and cell-matrix interactions. We show that the nano-scale topography of the extracellular
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Articoli

Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library

The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

Examination of Efficacy, Specificity and Efficiency of an shRNA Lentiviral Library

Introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

Protocolli

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.