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SAE0045

Sigma-Aldrich

HRV-3C Protease.

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

Sinonimo/i:

Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease

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About This Item

Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

human (human Rhinovirus Type 14)

Livello qualitativo

200

Saggio

≥90% (SDS-PAGE)

Forma fisica

aqueous solution

Attività specifica

≥5000 U/mg

PM

21 kDa

Concentrazione

0.8-1.2 mg/mL

tecniche

protein purification: suitable

Compatibilità

suitable for protein modification

applicazioni

life science and biopharma

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.

Applicazioni

HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.

Definizione di unità

One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

Stato fisico

Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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