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Key Documents

SAB4500620

Sigma-Aldrich

Anti-DAPK1 antibody produced in rabbit

affinity isolated antibody

Sinonimo/i:

DAP kinase 1, DAPK, Death associated protein kinase, kinase DAPK1

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

PM

antigen 160 kDa

Reattività contro le specie

mouse, human

Concentrazione

~1 mg/mL

tecniche

ELISA: 1:1000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... DAPK1(1612)

Descrizione generale

Anti-DAPK1 Antibody detects endogenous levels of total DAPK1 protein.
Death associated protein kinase 1 (DAPK1) is a calcium/calmodulin–regulated (CaM-regulated) protein kinase that belongs to the kinases family. The protein structure contains a kinase domain, a CaM binding domain, eight ankyrin repeats, and a death domain. DAPK1 protein is encoded by the gene mapped to human chromosome 9q21.33. Anti-DAPK1 antibody detects endogenous levels of total DAPK1 protein.

Immunogeno

The antiserum was produced against synthesized peptide derived from human DAPK1.

Immunogen Range: 274-323

Applicazioni

Anti-DAPK1 antibody produced in rabbit has been used in:
  • immunohistochemistry(1:400 )
  • coimmunoprecipitation
  • immunoblot

Azioni biochim/fisiol

Death associated protein kinase 1 (DAPK1) acts as a death-inducing factor in response to interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and transforming growth factor (TGF)-β. It also induces autophagy, proliferation, and immune response to inflammatory signals. DAPK1 promotes the growth of p53-mutant cancers, including pancreatic and ovarian cancers. Therefore, downregulation of DAPK1 can be a potential therapeutic strategy for treating P53 mutant cancers. Overexpression of DAPK1 protein has been observed in Alzheimer′s disease (AD) patients.

Caratteristiche e vantaggi

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Stato fisico

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Asia Asiaf et al.
Asian Pacific journal of cancer prevention : APJCP, 20(3), 839-848 (2019-03-27)
Aims: Death-associated protein kinase-1 (DAPK1) is a pro-apoptotic Ser/Thr kinase that participates in cell apoptosis and tumor suppression. DAPK1 is frequently lost in many different tumor types including breast cancer. The aim of this study was to evaluate the promoter
Jing Zhang et al.
Cellular & molecular immunology, 11(3), 245-252 (2014-02-18)
Interferon regulatory factor (IRF) 7 has been demonstrated to be a master regulator of virus-induced type I interferon production (IFN), and it plays a central role in the innate immune response against viruses. Here, we identified death-associated protein kinase 1
Craig Stevens et al.
The Journal of biological chemistry, 284(1), 334-344 (2008-11-01)
Death-associated protein kinase (DAPK) is a multidomain enzyme that plays a central role in autophagic and apoptotic signaling, although the protein-protein interactions regulating DAPK functions are not well defined. Peptide aptamer libraries were used to identify the tumor suppressor protein
Jing Zhao et al.
The Journal of clinical investigation, 125(7), 2707-2720 (2015-06-16)
Estrogen receptor-negative (ER-negative) breast cancers are extremely aggressive and associated with poor prognosis. In particular, effective treatment strategies are limited for patients diagnosed with triple receptor-negative breast cancer (TNBC), which also carries the worst prognosis of all forms of breast

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