SAB4200785
Anti-B2M antibody, Mouse monoclonal
clone 92A, purified from hybridoma cell culture
Sinonimo/i:
Anti-Beta-2-microglobulin
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified from hybridoma cell culture
Tipo di anticorpo
primary antibodies
Clone
92A, monoclonal
Stato
buffered aqueous solution
PM
~15 kDa
Reattività contro le specie
human
Concentrazione
~1 mg/mL
tecniche
immunoblotting: 0.1-0.2 μg/mL using β2-Microglobulin from human urine.
immunoprecipitation (IP): 2.5-5 μg/test using lysate of human HeLa cell line.
indirect ELISA: 0.2-0.4 μg/mL using 1 μg/ml β2-Microglobulin from human urine for coating.
Isotipo
IgG2a
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... B2M(567)
Descrizione generale
B2M (β2-Microglobulin) is the light chain of the major histocompatibility class (MHC) I molecule expressed on the cell surface of all nucleated cells, including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells and is absent in erythrocytes. B2M is filtered during glomerular filtrations and re-absorbed and catabolized by proximal renal tubule. While under normal conditions the B2M is undetectable in urine, its increased urinary excretion has been observed to be an early marker of tubular injury in a number of settings, including nephrotoxicant exposure, cardiac surgery and renal transplantation, preceding rises in serum creatinine by as many as 4-5 days. B2M may also serve as an early biomarker for AKI (Acute Kidney Injury). B2M plays a vital role in cell survival, proliferation and metastasis in various types of cancer. Its levels are also linked with multiple myeloma (MM) and non-Hodgkin′s lymphoma (NHL). NHL patients showing higher levels of B2M have a poor disease prognosis and a higher mortality risk. The protein has a predominantly β-pleated sheet structure that can form amyloid fibrils in some pathological conditions. In patient subjected to long-term hemodialysis, B2M is responsible for systemic amyloidosis. The levels of this protein are also altered in the cerebrospinal fluid of patients with neurological diseases, such as leptomeningeal metastasis, purulent meningitis, viral meningitis or encephalitis and neuroborreliosis. Over-expression of serum B2M is observed in hepatitis C virus (HCV) related chronic liver diseases and thus acts as a potential marker for the HCV disease progression towards cirrhosis and carcinoma.
Immunogeno
Recombinant B2M protein.
Applicazioni
Immunoblotting: a working concentration of 0.1-0.2 μg/mL is recommended using β2-Microglobulin from human urine.
Immunoprecipitation: a working concentration of 2.5-5 μg/test is recommended using lysate of human HeLa cell line.
Indirect ELISA: a working concentration of 0.2-0.4 μg/mL is recommended using 1 μg/mL ß2-Microglobulin from human urine for coating.
Immunoprecipitation: a working concentration of 2.5-5 μg/test is recommended using lysate of human HeLa cell line.
Indirect ELISA: a working concentration of 0.2-0.4 μg/mL is recommended using 1 μg/mL ß2-Microglobulin from human urine for coating.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.
Altre note
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
M C Chapelsky et al.
Drug safety, 7(4), 304-309 (1992-07-01)
This study assessed the potential nephrotoxicity of clarithromycin in comparison with gentamicin and placebo. Increased urinary excretion of alanine aminopeptidase (AAP) and N-acetyl-beta-D-glucosaminidase (NAG) served as markers of renal tubular injury. The study utilised a multiple-dose, double-blind, randomised, parallel group
N E Tolkoff-Rubin et al.
Clinics in laboratory medicine, 8(3), 507-526 (1988-09-01)
Traditional methods of noninvasively evaluating patients for renal injury do not accomplish the following tasks: reliably distinguish potentially treatable forms of acute renal failure from acute tubular necrosis; provide a sensitive indicator of early allograft rejection in renal transplant recipients
Philip R Greipp et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 23(15), 3412-3420 (2005-04-06)
There is a need for a simple, reliable staging system for multiple myeloma that can be applied internationally for patient classification and stratification. Clinical and laboratory data were gathered on 10,750 previously untreated symptomatic myeloma patients from 17 institutions, including
Stefan Schaub et al.
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 5(4 Pt 1), 729-738 (2005-03-12)
Our aim is to develop noninvasive tests to monitor the renal allograft posttransplant. Previously, we have reported that an unbiased proteomic-based approach can detect urine protein peaks associated with acute tubulointerstitial renal allograft rejection. Identification of these proteins peaks by
M G Dehne et al.
Der Anaesthesist, 44(8), 545-551 (1995-08-01)
After cardiac surgery, transient renal dysfunction often occurs. Regional differentiation of these processes is possible only using invasive techniques, including renal biopsy. Approximately 30 different plasma protein components have been identified in the urine of healthy individuals by means of
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