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SAB4200732

Sigma-Aldrich

Anti-β-Tubulin I antibody, Mouse monoclonal

clone SAP.4G5, purified from hybridoma cell culture

Sinonimo/i:

Anti-TUBB

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

mouse

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

SAP.4G5, monoclonal

Stato

buffered aqueous solution

PM

~55 kDa

Reattività contro le specie

bovine, gerbil, Xenopus, human, canine, mouse, hamster, chicken, rat, monkey

Concentrazione

~1.0 mg/mL

tecniche

immunoblotting: 0.06-0.12 μg/mL using HeLa cell extract
immunofluorescence: 1-2.5 μg/mL using HeLa cells
immunohistochemistry: 1-2 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human fallopian tube or testis sections.

Isotipo

IgG1

N° accesso UniProt

P07437

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... TUBB(203068)

Descrizione generale

Monoclonal Anti-β-Tubulin I (mouse IgG1 isotype) is derived from the SAP.4G5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. Tubulin is the major building block of microtubules, an intracellular cylindrical filamentous structure, that is present in almost all eukaryotic cells. Tubulin exists in all cells as a 100 kDa protein, which assembles from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of 55 kDa and share considerable homology.

Immunogeno

synthetic peptide corresponding to the C-terminal sequence of ß-tubulin isotype I, conjugated to BSA

Applicazioni

Anti-β-Tubulin I antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Azioni biochim/fisiol

β-Tubulin I is also useful in deducing the role that different tubulin isotypes play in nerve development, function or maintenance.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
017M4766
51160506

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Brian Perry et al.
Journal of the Association for Research in Otolaryngology : JARO, 4(3), 329-338 (2003-12-24)
The seven mammalian isotypes of beta tubulin are strikingly similar in amino acid sequence. The differences in isotypic sequence, although small, are nonetheless conserved in evolution, which suggests that they may confer distinct functional roles. If so, such roles should
Tubulin modifications and their cellular functions
Hammond JW, et al.
Current Opinion in Cell Biology, 20(1), 71-76 (2008)
S S Siddiqui et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 9(8), 2963-2972 (1989-08-01)
We have identified 2 anti-tubulin monoclonal antibodies that bind to 2 different subpopulations of identified neurons in the simple nervous system of the nematode Caenorhabditis elegans. The antibodies also recognize specific tubulin isotypes from C. elegans that were separated by
M C Roach et al.
Cell motility and the cytoskeleton, 39(4), 273-285 (1998-05-15)
Tubulin, the subunit protein of microtubules, is an alpha/beta heterodimer. In many organisms, both alpha and beta consist of various isotypes. Although the isotypes differ in their tissue distributions, the question of whether the isotypes perform different functions in vivo

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