SAB4200637
Monoclonal Anti-phospho-RNA polymerase II CTD (pSer2) antibody produced in rat
clone 3E7C7, purified from hybridoma cell culture
Sinonimo/i:
POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1, polymerase (RNA) II (DNA directed) polypeptide A 220kDa (POLR2A)
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rat
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
3E7C7, monoclonal
PM
~250 kDa
Reattività contro le specie
rat, canine, mouse, human, monkey
Concentrazione
~1 mg/mL
tecniche
flow cytometry: 10 μg/test using using HeLa cells.
immunoblotting: 0.25-0.5 μg/mL using whole extracts of HeLa cells.
immunofluorescence: 4-8 μg/mL using using A549 cells
Isotipo
IgG2a
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
phosphorylation (pSer2)
Informazioni sul gene
human ... POLR2A(5430)
Descrizione generale
Monoclonal Anti-phospho-RNA polymerase II CTD (pSer2 ) (rat IgG2a isotype) is derived from the hybridoma 3E7C7 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with a synthetic peptide containing phospho-Ser2 of human RNA Polymerase II CTD repeat. RNA polymerase II subunit A (RNAPII), also known as POLR2A, is the largest subunit of RNA polymerase II. The POLR2A gene is mapped to human chromosome 17p13.1. This polymerase contains a carboxy-terminal domain (CTD) composed of heptapeptide repeats. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase.
Specificità
Monoclonal Anti-RNA polymerase II CTD (pSer2) recognizes specifically phospho-Ser2 RNAPII but not phosphor-Ser5 or phosphor-Ser7.
Immunogeno
synthetic peptide containing phospho-Ser2 of human RNA Polymerase II CTD repeat.
Applicazioni
Monoclonal Anti-phospho-RNA polymerase II CTD (pSer2) antibody produced in rat may be used in:
- immunoblotting
- immunofluorescence
- flow cytometry
Azioni biochim/fisiol
RNA polymerase II subunit A (RNAPII) is responsible for synthesizing messenger RNA in eukaryotes. The carboxy-terminal domain (CTD) is essential for polymerase activity. The CTD mediates the coupling of transcription and processing of the nascent RNA. It also plays roles in transcription, elongation and termination. Also, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. CTD is being dynamically modified during the transcription cycle. The phosphorylation of serine is needed for RNA capping and splicing factors recruitment.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stoccaggio e stabilità
For extended storage, freeze at -20 0C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not usedwithin 12 hours.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Jacob C Schwartz et al.
Genes & development, 26(24), 2690-2695 (2012-12-20)
Mutations in the RNA-binding protein FUS (fused in sarcoma)/TLS have been shown to cause the neurodegenerative disease amyotrophic lateral sclerosis (ALS), but the normal role of FUS is incompletely understood. We found that FUS binds the C-terminal domain (CTD) of
Jun Odawara et al.
BMC genomics, 12, 516-516 (2011-10-21)
Cellular function is regulated by the balance of stringently regulated amounts of mRNA. Previous reports revealed that RNA polymerase II (RNAPII), which transcribes mRNA, can be classified into the pausing state and the active transcription state according to the phosphorylation
Ballachanda N Devaiah et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(18), 6927-6932 (2012-04-18)
The bromodomain protein, BRD4, has been identified recently as a therapeutic target in acute myeloid leukemia, multiple myeloma, Burkitt's lymphoma, NUT midline carcinoma, colon cancer, and inflammatory disease; its loss is a prognostic signature for metastatic breast cancer. BRD4 also
Jing-Ping Hsin et al.
Genes & development, 26(19), 2119-2137 (2012-10-03)
The C-terminal domain (CTD) of the RNA polymerase II largest subunit consists of multiple heptad repeats (consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7), varying in number from 26 in yeast to 52 in vertebrates. The CTD functions to help couple transcription and processing of the
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