SAB4200631

Monoclonal Anti-CYBA antibody produced in mouse

clone CB-64, purified from hybridoma cell culture

• Sinonimo/i: Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, cytochrome b-245 alpha polypeptide, p22-PHOX
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: CB-64, monoclonal
• Forma fisica: buffered aqueous solution
• Reattività contro le specie: bovine, mouse, rat, monkey, human, canine
• Concentrazione: ~1 mg/mL
• tecniche: flow cytometry: 5-10 μg/test using HeLa cells.; immunoblotting: 0.5-1 μg/mL using whole extracts of HeLa cells.; immunofluorescence: 5-10 μg/mL using HeLa cells.
• Isotipo: IgM
• N° accesso UniProt: P13498
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CYBA(1535) ; mouse ... Cyba(13057) ; rat ... Cyba(79129)

Descrizione

Descrizione generale

Monoclonal Anti-CYBA (mouse IgM isotype) is derived from the hybridoma CB-64 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the internal region of human CYBA, conjugated to keyhole limpet hemocyanin (KLH). The small subunit, p22phox subunit, also known as cytochrome b245, alpha polypeptide/light chain (CYBA) is membrane bound,which is expressed in phagocytic, endothelial and vascular smooth muscle cells. This gene is located on human chromosome16q24

Immunogeno

Synthetic peptide corresponding to a sequence at the internal region of human CYBA .

Applicazioni

Monoclonal Anti-CYBA antibody produced in mouse has been used in:

• immunoblotting
• flow cytometry
• immunofluorescence

Azioni biochim/fisiol

In prostate cancer cells downregulation of cytochrome b245, alpha polypeptide/light chain (CYBA) was reported to inhibited cell proliferation and colony formation, through AKT and ERK1/2 signaling pathways, indicating CYBA has a role in tumor angiogenesis and tumor growth. Mutations in this gene causes an autosomal recessive forms of chronic granulomatous disease (AR220CGD).

Stato fisico

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable