Passa al contenuto
Merck
Tutte le immagini(3)

Key Documents

SAB4200477

Sigma-Aldrich

Anti-ALIX (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Sinonimo/i:

Anti-PDCD6IP, programmed cell death 6 interacting protein, AIP1, DRIP4, HP95, ALG-2 interacting protein 1, Anti-apoptosis-linked gene 2-interacting protein X, Anti-dopamine receptor interacting protein 4

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

PM

antigen ~95 kDa

Reattività contro le specie

human, mouse

Concentrazione

~1.0 mg/mL

tecniche

immunoprecipitation (IP): 1-2 μg using lysates of human Jurkat cells
indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells
western blot: 1-2 μg/mL using whole extracts of mouse A20 cells

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... PDCD6IP(10015)
mouse ... Pdcd6ip(18571)

Descrizione generale

The gene PDCD6IP (programmed cell death 6 interacting protein), also known as ALIX (apoptosis-linked gene 2-interacting protein X), is mapped to human chromosome 3p22.1 The protein has a crescent-shaped Bro1 domain, V-shaped V domain and a proline-rich region.

Specificità

Anti-ALIX (C-terminal) recognizes human and mouse ALIX.

Immunogeno

peptide corresponding to a sequence at the C-terminal region of human ALIX/ PDCD6IP, conjugated to KLH. The corresponding sequence is identical in mouse, pig, monkey, bovine and dog and differs by a single amino acid in rat.

Applicazioni

Anti-ALIX (C-terminal) antibody produced in rabbit may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Azioni biochim/fisiol

Apoptosis-linked gene 2-interacting protein X (ALIX) protein binds to endophilin proteins that regulate membrane shape during endocytosis. Over-expression of the ALIX gene and endophilin leads to cytoplasmic vacuolization, which may be a reason for protection against cell death.
PDCD6IP (programmed cell death 6 interacting protein) interacts with PDCD6, a protein involved in apoptosis. It also participates in intraluminal endosomal vesicle biogenesis, viral budding and cytokinetic abscission. PDCD6IP is a part of the ESCRT (endosomal sorting complexes required for transport) machinery.

Stato fisico

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Non trovi il prodotto giusto?  

Prova il nostro Motore di ricerca dei prodotti.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Association between Programmed Cell Death 6 Interacting Protein Insertion/Deletion Polymorphism and the Risk of Breast Cancer in a Sample of Iranian Population.
Hashemi M
Disease Markers (2015)
ALIX is recruited temporarily into HIV-1 budding sites at the end of gag assembly.
Ku PI
PLoS ONE, 9 (2014)
Heparanase activates the syndecan-syntenin-ALIX exosome pathway.
Roucourt B
Cell Research, 25, 412-428 (2015)
Jun-Chao Xie et al.
American journal of translational research, 10(1), 150-163 (2018-02-10)
Hypoxia promotes the accumulation of amyloid-β (Aβ), which is related to the pathogenesis of Alzheimer's disease (AD). CD147 is considered as an additional subunit of γ-secretase regulated by hypoxia, and has been identified in exosomes. Aβ is also found in
Junchao Xie et al.
Neurochemical research, 44(5), 1113-1126 (2019-02-17)
Hypoxia promotes both total extracellular and exosomal amyloid-β (Aβ) production and aggravates Alzheimer's disease (AD). Resveratrol (RSV) has been proved to be neuroprotective in AD models, and down-regulated the expression of CD147, an additional subunit of γ-secretase. In this study

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.