S7571

Superoxide Dismutase from bovine erythrocytes

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

• Sinonimo/i: CU/ZN-SOD, Superoxide Dismutase 1 bovine, cytocuprein, erythrocuprein, hemocuprein, SOD, Superoxide: superoxide oxidoreductase
• Numero CAS: 9054-89-1
• Classificazione EC (Enzyme Commission): 1.15.1.1 (BRENDA, IUBMB)
• Numero CE: 232-943-0
• Numero MDL: MFCD00132404
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: bovine
• Forma fisica: lyophilized powder
• Attività specifica: ≥3,000 units/mg protein
• PM: 32.5 kDa
• Composizione: Protein, ≥95% biuret
• Condizioni di stoccaggio: (Store under nitrogen.; Tightly closed. Dry.)
• Caratteristiche più verdi: Atom Economy; Design for Energy Efficiency; Use of Renewable Feedstocks; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• tecniche: immunoblotting: suitable; inhibition assay: suitable
• Colore: blue-green
• pI: 4.95
• Solubilità: water: 20 mg/mL; aqueous buffer, pH 7.5: soluble
• N° accesso UniProt: P00442; P41976
• applicazioni: diagnostic assay manufacturing
• Categoria alternativa più verde: , Re-engineered
• Temperatura di conservazione: −20°C
• Informazioni sul gene: cow ... SOD1(281495) , SOD2(281496)

Descrizione

Descrizione generale

Research area: Cell Signaling

Superoxide dismutase (SOD) is a redox-active metalloenzyme expressed in both aerobic and anaerobic living organisms. Bovine superoxide dismutase or CuZn SOD is a homodimer with each subunit containing one zinc and one copper ion.

Applicazioni

Superoxide dismutase from bovine erythrocytes has been used:

• in a study to assess a kinetic model of radiation-induced inactivation of superoxide dismutase in nitrous oxide-saturated solutions
• in a study to investigate the possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction
• to investigate its effect on the hemolysis rate of human RBCs and hemoglobin-nitric oxide complex (HbNO) stability in human erythrocytes
• in combination with catalase to study its effect on cell differentiation in vitro
• to quantify superoxide levels and study their effect on reactivity in mouse pulmonary arteries through chemiluminescence and cytochrome C reduction methods

Azioni biochim/fisiol

Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Definizione di unità

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 ml reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

Stato fisico

Lyophilized powder, essentially salt-free

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)