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G6137

Sigma-Aldrich

Glutathione Peroxidase from bovine erythrocytes

lyophilized powder, ≥300 units/mg protein

Sinonimo/i:

GSH-Px, Glutathione:hydrogen-peroxide oxido-reductase

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About This Item

Numero CAS:
9013-66-5
Classificazione EC (Enzyme Commission):
1.11.1.9 (BRENDA, IUBMB)
Numero CE:
232-749-6
Numero MDL:
MFCD00131195
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

bovine erythrocytes

Livello qualitativo

200

Forma fisica

lyophilized powder

Attività specifica

≥300 units/mg protein

PM

84.5 kDa

Composizione

Protein, 10-30% modified Warburg-Christian

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

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Descrizione generale

Glutathione peroxidase is an antioxidant enzyme that contains selenium. It is present in the glandular epithelium of human endometrium.

Applicazioni

Glutathione Peroxidase from bovine erythrocytes has been used as an oxygen radical scavenger to study its effect on cytotoxicity of 1,3-dilinoleoylglycerol (DLG) against E1A-3Y1 cells.
Glutathione peroxidase from bovine erythrocytes was used as a positive control in cloning and characterization of full-length cDNAs encoding two glutathione peroxidases (GpXs) from Globodera rostochiensis. It was used for the determination of glutathione peroxidase activity in human milk.

Azioni biochim/fisiol

Glutathione peroxidase helps to reduce (peroxides) H2O2 to water and lipid peroxides to lipid alcohols.
Glutathione peroxidase is an enzyme which reduced lipid hydroperoxides into their corresponding alcohols. It also reduces free hydrogen peroxide in to water. In vivo it is responsible for protecting hemoglobin from oxidative breakdown.
Protects cells against oxidative damage by catalyzing the reduction of hydrogen peroxide in the presence of reducing agent glutathione. In cellular membranes it may induce lipid peroxidation through the reduction of hydrogen peroxide or polyunsaturated fatty acid hydroperoxides.

Definizione di unità

One unit will catalyze the oxidation by H2O2 of 1.0 μmole of reduced glutathione to oxidized glutathione per min at pH 7.0 at 25 °C.

Stato fisico

Lyophilized powder containing 25% sucrose and 2.5% dithiothreitol with sodium phosphate buffer salts

Altre note

Note: At the reported pH optimum of 8.8, we have found the activity to be approx. 10 times that at pH 7.0. However, to remain consistent with literature and avoid complications arising from non-enzymatic oxidation of GSH, our unit is defined at pH 7.0.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Superoxide Dismutase from bovine erythrocytes BioReagent, ≥3,000 units/mg protein, lyophilized powder, suitable for cell culture

Sigma-Aldrich

S5395

Superoxide Dismutase from bovine erythrocytes

Superoxide Dismutase from bovine erythrocytes lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

Sigma-Aldrich

S7571

Superoxide Dismutase from bovine erythrocytes

Cumene hydroperoxide technical grade, 80%

Sigma-Aldrich

247502

Cumene hydroperoxide

Yue Wang et al.
Analytical chemistry, 92(2), 1997-2004 (2019-12-21)
Solid evidence confirms that glutathione peroxidase (GPx) is a kind of vital protease in the first-line antioxidant defense system and participates in regulation of redox homeostasis as well as the pentose phosphate pathway. However, the current methods cannot achieve real-time
Hemoglobin catabolism. I. Glutathione peroxidase, an erythrocyte enzyme which protects hemoglobin from oxidative breakdown.
G C MILLS
The Journal of biological chemistry, 229(1), 189-197 (1957-11-01)
O Werz et al.
European journal of biochemistry, 242(1), 90-97 (1996-11-15)
Differentiation of HL-60 cells by dimethylsulfoxide induces 5-lipoxygenase protein expression, but only low cellular 5-lipoxygenase activity. Similarly, B-lymphocytes express 5-lipoxygenase protein and show activity in cell homogenates but not in intact cells. Here, we demonstrate that suppression of cellular 5-lipoxygenase
The role of oxidative stress in endometriosis
Handbook of Fertility, 273-281 (2015)
Angeles Torres et al.
Die Nahrung, 47(6), 430-433 (2004-01-20)
An analytical method for determining glutathione peroxidase (GPx) (EC 1.11.1.9) activity in whole blood has been adapted to human milk samples. The values obtained for precision (relative standard deviation: 8.4%), linearity and accuracy (recovery: 90.4%) indicate the adequacy of the
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