S7025

50 bp DNA Step Ladder

for DNA electrophoresis

• Sinonimo/i: DNA Ladder, Step Ladder, 50 bp, 17 Fragments, 50-3000 bp
• Codice UNSPSC: 41105335
• NACRES: NA.25

Proprietà

• Livello qualitativo: 100
• Stato: liquid
• impiego: 100 uses
• Compatibilità: suitable for electrophoresis (DNA)
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Sigma′s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting.

Applicazioni

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Componenti

Sigma′s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA.

Altre note

Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands).

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable