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D3812

Sigma-Aldrich

DirectLoad 50 bp DNA Step Ladder

ready-to-use marker for DNA electrophoresis

Sinonimo/i:

DirectLoad 50 bp DNA Marker, Ready-to-use 50 bp DNA Marker, Ready-to-use DirectLoad 50 bp DNA Marker, Ready-to-use DirectLoad 50 bp DNA Step Ladder

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About This Item

Codice UNSPSC:
41105335
NACRES:
NA.25

Livello qualitativo

Forma fisica

(liquid)

impiego

sufficient for 100 loads

Compatibilità

suitable for electrophoresis (DNA)

Temperatura di conservazione

−20°C

Descrizione generale

Sigma′s DirectLoad 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Applicazioni

DirectLoad 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Caratteristiche e vantaggi

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Componenti

Sigma′s DirectLoad 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Altre note

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Note legali

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Prodotti correlati

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Protocolli

When using hot start Taq DNA polymerase, the enzyme remains inactive until heated. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme.

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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