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Key Documents

S1265

Sigma-Aldrich

Anti-Sheep IgG (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

rabbit

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

IgG fraction of antiserum

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

tecniche

indirect ELISA: 1:50,000-1:100,000

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Descrizione generale

Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other rabbit serum proteins. Antiserum is determined to be immunospecific for sheep IgG by immunoelectrophoresis (IEP) versus sheep IgG and normal sheep serum. Identity and purity of the antibody is established by immunoelectrophoresis (IEP). Electrophoresis of the product followed by diffusion versus the anti-rabbit IgG and the anti-rabbit whole serum result in single arcs of precipitation in the γ region.

Specificità

Binds all sheep Igs

Immunogeno

purified sheep IgG

Applicazioni

Anti-Sheep IgG (whole molecule) antibody produced in rabbit has been used in:
  • Enzyme-linked immunosorbent assay (ELISA)
  • Immunoprecipitation
  • labelling electrodes in immunosensor systems

Azioni biochim/fisiol

Digestion of IgG by papain generates fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallisable (fc). IgG antibody has enormous therapeutic potential and the Fc is involved in the development of therapeutic antibody.

Stato fisico

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as preservative.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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