S0266

Spermidine

BioReagent, for molecular biology, suitable for cell culture, ≥98%

• Sinonimo/i: 1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane
• Formula condensata: NH₂(CH₂)₃NH(CH₂)₄NH₂
• Numero CAS: 124-20-9
• Peso molecolare: 145.25
• Beilstein: 1698591
• Numero CE: 204-689-0
• Numero MDL: MFCD00008229
• Codice UNSPSC: 12352200
• ID PubChem: 24899434
• NACRES: NA.52

Proprietà

• Grado: Molecular Biology; for molecular biology
• Nome Commerciale: BioReagent
• Saggio: ≥98%
• Stato: solid or liquid
• PM: 145.25 (AMU)
• tecniche: cell culture | mammalian: suitable
• Indice di rifrazione: n20/D 1.479 (lit.)
• Densità: 0.925 g/mL at 25 °C (lit.)
• Attività estranea: DNase, RNase, and protease, none detected
• Temperatura di conservazione: room temp
• Stringa SMILE: NCCCCNCCCN
• InChI: 1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2; ATHGHQPFGPMSJY-UHFFFAOYSA-N
• Informazioni sul gene: rat ... Ppm1a(24666)

Descrizione

Descrizione generale

Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.

Applicazioni

Spermidine has been used in the preparation of gold beads and as a component in nuclei isolation buffer (NIB).

Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.

Azioni biochim/fisiol

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T₄ polynucleotide kinase activity.

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T₄ polynucleotide kinase activity.

Spermidine helps to maintain the proper activity of gene expression. This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300). Spermidine participates in cell growth.

Avvertenza

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H314
• Consigli di prudenza: P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405
• Classi di pericolo: Eye Dam. 1 - Skin Corr. 1B
• Codice della classe di stoccaggio: 8A - Combustible corrosive hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): 233.6 °F - closed cup
• Punto d’infiammabilità (°C): 112 °C - closed cup
• Dispositivi di protezione individuale: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter