R9894
Ras human
Wild type, recombinant, expressed in E. coli, buffered aqueous glycerol solution
Sinonimo/i:
H-Ras, rH-RAS, WT
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About This Item
Prodotti consigliati
Ricombinante
expressed in E. coli
Livello qualitativo
Saggio
≥80% (SDS-PAGE)
Stato
buffered aqueous glycerol solution
PM
28 kDa
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Informazioni sul gene
human ... RASGEF1B(153020)
Descrizione generale
Ras proto-oncogene is a guanine-nucleotide binding protein that couples tyrosine kinase receptor signals to mitogen activated protein (MAP) kinase cascade. It has an important role in cell growth. The Ras gene is localized on human chromosome 11p15.5. Ras is a substrate for farnesyltransferase (FTase) and when farnesylated, it is anchored to the membrane.
Applicazioni
Human Ras has been used as a negative control in tubulin overlay assay.
Azioni biochim/fisiol
Ras proteins, p21, are guanyl nucleotide binding proteins that play an important role in cellular signal transduction. Ras proteins are regulatory proteins that bind GTP in their active state. A Ras intrinsic GTPase activity hydrolyzes guanosine-5′-triphosphate (GTP) to guanosine diphosphate (GDP) thus inactivating Ras, while activation of Ras requires exchange of the bound GDP with GTP. Inhibition of Ras activity by stimulation of its GTPase activity is enhanced by GTPase activating proteins. Membrane localization of Ras is crucial for its cellular activity. Inhibition of Ras farnesylation is a target for anti-cancer drug development, since abnormally active Ras is present in more than 50% of human cancers.
Confezionamento
Package size based on protein content
Stato fisico
Solution in 50% glycerol containing 50 mM Tris (pH 8.0), 5 mM MgCl2, 45 mM NaCl, 1 mM DTT
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Human Mucin Gene MUC5B, the 10.7-kb Large Central Exon Encodes Various Alternate Subdomains Resulting in a Super-repeat
Jean-Luc D
The Journal of Biological Chemistry (1997)
Slow conformational dynamics of the guanine nucleotide-binding protein Ras complexed with the GTP analogue GTPgammaS.
Spoerner M
FEBS Journal (2007)
J Tucker et al.
The EMBO journal, 5(6), 1351-1358 (1986-06-01)
v-Ha-ras encoded p21 protein (p21V), the cellular c-Ha-ras encoded protein (p21C) and its T24 mutant form p21T were produced in Escherichia coli under the control of the tac promoter. Large amounts of the authentic proteins in a soluble form can
Real-time in vitro measurement of intrinsic and Ras GAP-mediated GTP hydrolysi
Shutes A, et al.
Test (2006)
Structure and function of p21 ras proteins
Shih TY, et al.
Gene Analysis Techniques (1986)
Articoli
We present an article about how proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication.
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