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Documenti fondamentali

R7256

Sigma-Aldrich

RPMI 1640 Vitamins Solution (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Sinonimo/i:

RPMI 1640 Solution, RPMI 1640 Vitamins, Vitamins Solution

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About This Item

Codice UNSPSC:
12352205
NACRES:
NA.75

Sterilità

sterile-filtered

Livello qualitativo

Nome Commerciale

BioReagent

Stato

liquid

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Colore

yellow to dark yellow

pH

7.2-7.6

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

Roswell Park Memorial Institute medium is commonly referred as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. RPMI 1640 is used for the serum-free expansion of human lymphoid cells.

Applicazioni

RPMI 1640 Vitamins Solution (100×) has been used:
  • in the preparation of CeM1 medium for the isolation of Caenorhabditis elegans germ cells
  • as a component of the amino-acid-deprived medium for the starvation of intestinal porcine enterocytes
  • as a component of the stage-8 medium for culturing of human pluripotent stem cell-derived 3D pancreatic progenitor clusters
RPMI 1640 Vitamins Solution (100×) is used as a supplement in cell culture media.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Journal of structural biology, 183(3), 467-473 (2013-07-03)
The stochastic nature of biological systems makes the study of individual cells a necessity in systems biology. Yet, handling and disruption of single cells and the analysis of the relatively low concentrations of their protein components still challenges available techniques.
Alexandra S Vagasi et al.
Bio-protocol, 7(15) (2017-09-05)
The Caenorhabditis elegans germ line is an important model system for the study of germ stem cells. Wild-type C. elegans germ cells are syncytial and therefore cannot be isolated in in vitro cultures. In contrast, the germ cells from tumorous

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