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Millipore

ProteoPrep® Total Extraction Sample Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Forma fisica

dry powder

Livello qualitativo

Temperatura di conservazione

2-8°C

Descrizione generale

ProteoPrep® Total Extraction Sample Kit is designed to extract all proteins from any type of cell or tissue. It includes two reagents that have been used extensively for extraction and two new reagents with increased solubilizing power to extract more proteins. This kit also includes reagents for the reduction and alkylation of disulfide bonds. This allows comparison of the protein extractions obtained with each of the four reagents and optimization to meet your individual needs. The special reducing and alkylating reagents produce protein samples that exhibit improved focusing and decreased streaking in 2D gels.

Applicazioni

ProteoPrep® Total Extraction Sample Kit has been used to extract total protein from various sources such as epidermal stem cells and rheumatoid arthritis (RA) synovial fibroblasts.

Caratteristiche e vantaggi

  • Four pre-mixed solubilization reagents - Enables rapid solubilization.
  • Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
  • Innovative detergent preparations - Highly improved solubility allows higher protein loads and greater visibility of low abundance proteins in 2D gels.

Stoccaggio e stabilità

These reagents should remain stable for at least 6 months in their unopened container.

Altre note

The ProteoPrep® Total Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.
This kit contains enough reagents to generate at least ten 2-ml samples using all four reagents. For researchers who have optimized an extraction protocol using one chaotropic extraction reagent, each reagent is available as an individual product as well.

Note legali

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Samples containing high amount of salts and buffers may not work with this kit. These samples should be dialyzed prior to use. This product is for R&D use only, not for drug, household, or other uses.

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS

  • C0481Protein Extraction Reagent Type 1SDS

  • C0606Protein Extraction Reagent Type 2SDS

  • C0731Protein Extraction Reagent Type 3SDS

  • C0356Protein Extraction Reagent Type 4SDS

  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56SDS

  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5SDS

Avvertenze

Danger

Classi di pericolo

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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