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PKH26PCL

Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling

Distributed for Phanos Technologies

Sinonimo/i:

Phagocytic cell label

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.32

Applicazioni

This kit is for phagocytic cell labeling. It is used to selectively label cells with phagocytic capabilities such as monocytes, macrophages or neutrophils.

Principio

The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for at least 21 days in vivo.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Note legali

CellVue is a registered trademark of Phanos Technologies

Solo come componenti del kit

N° Catalogo
Descrizione
  • Diluent B 6 x 10
  • PKH26 cell linker in ethanol .5 mL

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

FlameExclamation mark
GHS02,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H225,H319

Classi di pericolo

Eye Irrit. 2 - Flam. Liq. 2

Codice della classe di stoccaggio

3 - Flammable liquids

Punto d’infiammabilità (°F)

57.2 °F - closed cup

Punto d’infiammabilità (°C)

14.0 °C - closed cup

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Certificati d'analisi (COA)

Lot/Batch Number
MKCK2680
SLBW5928
SLBV4711
SLBR8270V
SLBL6374V

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Hyung-Syup Kim et al.
Journal of Korean Neurosurgical Society, 44(4), 249-255 (2008-12-20)
In Moyamoya disease, the primary goal of treatment is to improve collateral circulation through angiogenesis. In the present study, we obtained and sub-cultured bone marrow stromal cells (BMSCs) from rats without a cell-mediated immune response. Then, we injected the labeled
Jaime Murphy et al.
American journal of respiratory cell and molecular biology, 38(4), 380-385 (2008-01-15)
To further examine the half-life of alveolar macrophages, chimeric CD 45.2 mice were generated through bone marrow transplantation of donor CD 45.1 cells. Before administration of donor cells, recipient mice were divided into two cohorts: the first cohort received total
K Tabata et al.
Gene therapy, 18(10), 969-978 (2011-04-23)
We previously identified the mouse and human Glipr1 and GLIPR1/RTVP-1 genes, respectively, as direct p53 targets with proapoptotic activities in various cancer cell lines, including prostate cancer (PCa). Intratumoral injection of an adenoviral vector capable of efficient transduction and expression
Lara Campana et al.
Journal of immunology (Baltimore, Md. : 1950), 200(3), 1169-1187 (2017-12-22)
The disposal of apoptotic bodies by professional phagocytes is crucial to effective inflammation resolution. Our ability to improve the disposal of apoptotic bodies by professional phagocytes is impaired by a limited understanding of the molecular mechanisms that regulate the engulfment
Meaghan M Hunter et al.
Gastroenterology, 138(4), 1395-1405 (2010-01-05)
Infection with the rat tapeworm Hymenolepis diminuta reduces the severity of dinitrobenzene sulfonic acid (DNBS)-induced colitis in mice. Infection with H. diminuta increases colonic expression of arginase-1 and found in inflammatory zone 1 (FIZZ1), markers of alternatively activated macrophages (AAMs).
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