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Key Documents

P6998

Sigma-Aldrich

Protein Kinase A Catalytic Subunit β, Active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

Sinonimo/i:

PKA Catalytic Subunit β, Active human, PKA-Cβ), cAMP-dependent protein kinase

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About This Item

Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
12352202
NACRES:
NA.77

Ricombinante

expressed in baculovirus infected Sf9 cells

Livello qualitativo

Saggio

≥80% (SDS-PAGE)

Forma fisica

buffered aqueous glycerol solution

Attività specifica

≥10 nmol/min·mg

PM

protein apparent mol wt ~65 kDa (including N-terminal GST-tag of ~26kDa)

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... PRKACB(5567)

Descrizione generale

Protein kinase cAMP-activated catalytic subunit β (PRKACB) is one of the catalytic subunits of the serine/threonine kinase, protein kinase A. The gene encoding it is localized on human chromosome 1p36.1.

The molecular weight of about 65kDa is confirmed by the SDS-PAGE gel. The product was generated by expressing recombinant full-length human PKAcβ2 with an additional N-terminal GST tag by baculovirus in Sf9 insect cells. The gene accession number is NM_002731. The resulting protein is 351aa cAMP-dependent protein kinase catalytic subunit beta isoform 2 (NP_002722), or according to UniProt, Isoform 1 (P22694-1).

Applicazioni

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.

Azioni biochim/fisiol

cAMP-dependent protein kinase (PKA; EC 2.7.1.37) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription.

Definizione di unità

One unit will incorporate one nanomole of phosphate into the Crebtide (KRREILSRRPSYR) substrate per minute at 30 deg C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

Stato fisico

Supplied as a solution of 5 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Association between PKA gene polymorphism and NTDs in high risk Chinese population in Shanxi.
Wu J, et.al
International Journal of Clinical and Experimental Pathology, 6(12), 2968-2974 (2013)
Jian Wu et al.
International journal of clinical and experimental pathology, 6(12), 2968-2974 (2013-12-03)
This study aimed to investigate the single nucleotide polymorphisms (SNPs) of PKA and neural tube defects (NTDs) in Chinese population. A total of 183 NTDs cases and 200 healthy controls were used in this study. 7 selected single nucleotide polymorphism

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